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General information

Offer title : PhD thesis in molecular biology (M/F)
Reference : UMR9004-KONBRO-001
Number of position : 1
Workplace : MONTPELLIER
Date of publication : 17 February 2023
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 1 September 2023
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly
Section(s) CN : Molecular and structural biology, biochemistry

Description of the thesis topic

Regulation of gene transcription is the principal mechanism underlying appearance of the drug-tolerant, dormant, forms of bacteria and the chronic latent infections like tuberculosis. Current project aims to understand molecular mechanisms of transcription termination in the human pathogen, Mycobacterium tuberculosis and its impact on antibiotic resistance.
The study will be focused on the structure-functional analysis of the transcriptional factors (Tfs) binding to RNA polymerase (RNAP) of Mycobacterium tuberculosis. An objective of the project is to identify novel partners binding to Tfs and RNAP by using proteomics approaches. In addition, function of these factors in elongation and termination of transcription will be explored using a combination of biochemical methods, genetics and fluorescent reporters in the live cells.

Work Context

The thesis will be carried out in the team of Konstantin Brodolin at the Infectious Diseases Research Institute of Montpellier (IRIM, Montpellier, France). IRIM is located in the CNRS - University of Montpellier campus which offers a rich and dynamic scientific environment. The team explores molecular mechanisms of transcription regulation in pathogenic bacteria using biochemical, biophysical and structural approaches.

Constraints and risks

Use of radioactivity (isotope P-32)

Application Deadline : 18 May 2023

APPLY : https://emploi.cnrs.fr/Offres/Doctorant/UMR9004-KONBRO-001/Default.aspx?lang=EN